Figure 3.

Depletion of plasmacytoid dendritic cells (pDCs) results in increased Th2 inflammation after transfer of BMDCs pulsed with CSE, C. pneumoniae, or both. Mice were injected intravenously with either isotype control Ab (IgG2a, open bars), or with 120G8 Ab (solid bars), that is, the specific Ab for the depletion of pDCs. The intravenous injection of IgG2a or 120G8 Ab was performed 3 days after the adoptive transfer of CSE-pulsed and/or C. pneumoniae–pulsed BMDCs. The bronchoalveolar lavage (BAL) cell numbers (A) and eosinophil numbers (B) were evaluated as described in the text. The ELISA experiments were performed to evaluate IgE (C) and MCP-1 (D) in BAL-derived supernatants. Data represent mean ± SEM; n = 9 and n = 6 for pDC depletion experiments. Two separate experiments were performed. Statistically significant differences are denoted as *P < 0.05 and **P < 0.01, as determined by one-way ANOVA and the Student t test.