Figure 4.
Depletion of pDCs results in increased mDCs in the lung. The BMDCs were treated overnight with PBS, CSE (10%), C. pneumoniae (5 × 105 IFUs/ml), or both, and then adoptively transferred (intratracheally; 5 × 105 cells/mouse) into wild-type recipient mice. Mice were killed 5 days later, and lungs were excised and properly stained for CD11c-PE and CD11b-FITC. (A, B) Lung mDCs were expressed as percentages of CD11c highly positive CD11b intermediately positive cells. (A) An experiment representative of bar graph in B. Data represent mean ± SEM, n = 9 per group. Experiments were performed on 2 different days. Statistically significant differences are denoted as *P < 0.05, **P < 0.01, and ***P < 0.0001, as determined by one-way ANOVA and the Student t test.