Figure 5.

Lung CD11c⁺ cell release increased IL-5 ex vivo when pDCs were depleted before purification. The BMDCs were treated overnight with PBS, CSE (10%), C. pneumoniae (5 × 10⁵ IFUs/ml) or both, and then adoptively transferred (intratracheally; 5 × 10⁵ cells/mouse) into wild-type recipient mice. Two days later, 120G8 Ab or isotype control were intravenously injected (150μg/ml) lung CD11c⁺ cells were isolated by means of a technique using Miltenyi Biotec microbeads 3 days later. (A, B) IL-5 concentrations were measured using ELISA. Neutralizing Ab for IFN-α (2.5 ng/ml) or isotype control was added to CD11c⁺ cells isolated from the lung, and IL-5 concentrations were measured when exposed to CSE and C. pneumoniae (C, D). Concentrations of IL-5 were analyzed using ELISA in cell-free supernatants. Data represent mean ± SEM, n = 9 and n = 6 for pDC depletion experiments. Experiments were performed on 2 different days. Statistically significant differences are denoted as *P < 0.05, **P < 0.01, and ***P < 0.0001, as determined by one-way ANOVA and the Student t test.