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Airway hyperresponsiveness to methacholine. (A) On Day 33, AHR to methacholine (Mch) was established using a noninvasive, unrestrained, whole-body plethysmograph. (B) Airway hyperresponsiveness was validated by invasive tracheostomy and by measuring specific airway resistance (RL) to Mch. Data are shown as mean ± SEM for five mice in each group. *P < 0.01. **P < 0.001.
