TABLE 2.
CYTOKINE PROFILES FROM BRONCHOALVEOLAR FLUID OF WILD-TYPE AND CC10-CAIKKβ MICE AFTER 1 WEEK OF NORMAL OR DOX CHOW AND AIR OR EXPOSURE TO 25 ppm NO2
| Air |
NO2 |
Dox Air |
Dox NO2 |
|||||
|---|---|---|---|---|---|---|---|---|
| WT | CAIKKβ | WT | CAIKKβ | WT | CAIKKβ | WT | CAIKKβ | |
| IL-5 | 0.3 ± 0.3 | 1.4 ± 0.07 | 18.2 ± 2.5* | 12.0 ± 2.5* | 3.4 ± 2.1 | 4.3 ± 1.8 | 12.9 ± 3.0 | 2.8 ± 0.7 |
| IL-6 | 6.7 ± 6.7 | 21.6 ± 1.8 | 29.9 ± 1.5* | 29.9 ± 0.6* | 16.3 ± 0.9 | 30.7 ± 4.2* | 25.5 ± 31 | 29.9 ± 1.5 |
| IL-9 | 21.9 ± 3.5 | 14.9 ± 5.9 | 148.4 ± 56.0* | 141.6 ± 43.6* | 32.0 ± 4.3 | 21.4 ± 2.6 | 60.6 ± 30.0 | 31.2 ± 8.3 |
| IL-10 | 15.3 ± 4.7 | 18.0 ± 7.4 | 102.8 ± 35.0* | 100.0 ± 23.2* | 23.5 ± 1.4 | 23.1 ± 5.0 | 51.6 ± 14.4 | 24.5 ± 5.0 |
| IL-12 (p40) | 33.5 ± 6.0 | 38.7 ± 3.8 | 98.1 ± 3.4* | 84.3 ± 9.4* | 38.1 ± 7.3 | 119.8 ± 24.5‡ | 79.8 ± 10.1 | 166.8 ± 21.8† |
| G-CSF | 3.2 ± 0.8 | 4.9 ± 1.5 | 28.8 ± 3.5* | 34.9 ± 0.9* | 4.5 ± 1.2 | 48.4 ± 23.7‡ | 26.2 ± 10.0 | 37.3 ± 4.0 |
| KC | 19.8 ± 3.1 | 26.1 ± 4.0 | 34.8 ± 5.4* | 40.7 ± 3.0* | 16.2 ± 2.6 | 64.8 ± 15.0‡ | 22.3 ± 3.9 | 55.4 ± 7.5 |
| MCP-1 | 1.1 ± 1.1 | 4.0 ± 2.0 | 32.7 ± 5.8* | 32.2 ± 2.6* | 14.2 ± 3.9 | 27.9 ± 6.5‡ | 15.4 ± 5.4 | 53.2 ± 11.0† |
| MIP-1β | 0.0 ± 0.0 | 0.0 ± 0.0 | 8.9 ± 1.0* | 15.2 ± 2.6* | 0.0 ± 0.0 | 11.8 ± 4.7‡ | 5.2 ± 0.2 | 10.8 ± 2.3 |
| RANTES | 9.0 ± 1.3 | 9.4 ± 1.4 | 21.6 ± 1.6* | 24.5 ± 1.7* | 11.3 ± 1.7 | 45.5 ± 16.0‡ | 20.2 ± 2.0 | 43.2 ± 4.6 |
| TNF-α | 8.1 ± 4.1 | 7.8 ± 3.9 | 14.2 ± 1.5 | 23.9 ± 1.0 | 9.5 ± 3.9 | 12.9 ± 1.8 | 6.6 ± 2.3 | 13.9 ± 2.1 |
Definition of abbreviations: G-CSF, granulocyte colony–stimulating factor; Dox, doxycycline; KC, keratinocyte-derived chemoattractant; MCP-1, macrophage chemotactic protein; RANTES, regulated upon activation normal T-cell expressed and presumably secreted; WT, wild-type.
Mice received regular or Dox-containing food (Dox) for 3 days before inhalation of 25 ppm NO2 for 6 hours/day for an additional 3 days. All groups were analyzed on Day 7. Concentrations of IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-12 (p70), IL-13, IL-17, GM-CSF, MIP-1α, eotaxin, and IFNγ were below the limits of detection in all samples. Data represent cytokine levels in pg/ml, and are expressed as mean ± SEM from 4–6 mice/group.
P ≤ 0.05 compared with air control groups.
P ≤ 0.05 compared with WT Dox NO2 group.
P ≤ 0.05 compared with WT Dox air group.