TABLE 2.
PRIMARY ANTIBODIES USED IN THIS STUDY
| Antibody | Species | Source, Cat. No.* | Dilution |
|---|---|---|---|
| ChAT | goat | Chemicon, AB144 | 1:50 |
| VIP | Rabbit | Peninsula, IHC7161 | 1:200 |
| Nitric oxide synthase | Rabbit | Chemicon, AB5380 | 1:2,500 |
| Neuropeptide Y | Rabbit | Chemicon, AB1915 | 1:500 |
| Tyrosine hydoxylase | Rabbit | Chemicon, AB5986 | 1:1,000 |
| Substance P | Rabbit | Peninsula, AB7451 | 1:200 |
| Substance P | Rat | Peninsula, MAB356 | 1:200 |
| PGP9.5 | Rabbit | Ultraclone, PGP101 | 1:400 |
| PGP9.5 | Mouse | Ultraclone, 1C34 | 1:400 |
| Neurotrophin-3 | Rabbit | Santa Cruz, SC-547 | 1:500 |
Definition of abbreviations: ChAT, choline acetyltransferase; PGP, protein gene product; VIP, vasoactive intestinal peptide.
*
Chemicon International, Inc. (Temecula, CA); Peninsula Laboratories (Belmont, CA); Ultraclone (Isle of Wight, UK); Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).