Table 1. Reestimated parameter values for our previous models of mitochondrial Ca²⁺ uniporter with additional constraint: α _e = α _x = α≥0.

Parameters	Model 1		Model 2		References
	Case 1	Case 2	Case 1	Case 2
	44.50.300.34	44.50.300.34	32.60.380.43	32.60.380.43	[16] [13] [12]
	44.50.300.34	44.5×m20.30×m20.34×m2	32.60.380.43	32.6×m20.38×m20.43×m2	[16] [13] [12]
	45.9×10−645.9×10−688.1×10−6	45.9×10−645.9×10−688.1×10−6	38.7×10−638.7×10−674.3×10−6	38.7×10−638.7×10−674.3×10−6	[16] [13] [12]
	45.9×10−645.9×10−688.1×10−6	45.9×10−6×m45.9×10−6×m88.1×10−6×m	38.7×10−638.7×10−674.3×10−6	38.7×10−6×m38.7×10−6×m74.3×10−6×m	[16] [13] [12]
α e = α x = α	0.0	0.0	0.0	0.0	[12], [13], [16]
β e	0.113	0.113	0.113	0.113	[12], [13], [16]
β x	0.887	0.887	0.887	0.887	[12], [13], [16]

The rate constants and are redefined here as and and are in the units of nmol/mg/s; the binding constants and are in the units of molar. The kinetic and biophysical parameters satisfy the kinetic and thermodynamic constraints: and as well as the additional constraint: α _e = α _x = α≥0. The Case 1 corresponds to and , while the Case 2 corresponds to and . Note that m is an arbitrary number indicating the existence of multiple estimates of the kinetic parameters and in Case 2 of the uniporter model.