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Binding of Citrulline within the Pockets of DR1001. IC50 values for Citrulline (black bars) or Arginine (open bars) substitutions at positions 1, 4, 6, 7, and 9 of the modified HA 217–236 peptide sequence. Values are shown on a logarithmic scale centered on 0.1, the threshold value for binding. Relative binding affinity (RBA) values were calculated as the IC50 value of the unsubstituted peptide divided by the IC50 value of the substituted peptide.
