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. 2010 Jul-Aug;3(4):266–274. doi: 10.4161/oxim.3.4.12859

Figure 2.

Figure 2

Effect of ifosfamide (IFO), propionyl-l-carnitine (PLC), D-carnitine-mildronate (DC-MD) and their combination on the levels of CoA-SH (A), acetyl-CoA (B) and acetyl-CoA/CoA-SH (C) in isolated rat heart mitochondria. Rats were randomly divided into 6 different groups of 10 animals each: Control, D-carnitine-mildronate (DC-MD, carnitine-depleted group), PLC (carnitine supplemented group), IFO, DC-MD plus IFO and PLC plus IFO. Carnitine depletion was induced in rats by daily intraperitoneal injection of DC (250 mg/kg/day) combined with MD (200 mg/kg/day) for 10 successive days. Carnitine supplementation was induced in rats by daily intraperitoneal injection of PLC (250 mg/kg/day) for 10 successive days. Fanconi Syndrome was induced in rats by administration of IFO (50 mg/kg/day, I.P.) for 5 successive days. IFO-carnitine depleted rats were given the same doses of DC-MD for 5 days before and 5 days concomitant with IFO. IFO-carnitine supplemented rats were given the same doses of PLC for 5 days before and 5 days concomitant with IFO. At the end of the treatment protocol, rat hear mitochondria was isolated for measurement of CoA-SH, acetyl-CoA and CoA-SH/acetyl-CoA ratio. Data are presented as mean ± S.E.M. (n = 10). *, # and $ indicate significant change from control, IFO and DC-MD respectively, at p < 0.05 using ANOVA followed by Tukey-Kramer as a post ANOVA test.