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. 2010 Mar-Apr;3(2):122–128. doi: 10.4161/oxim.3.2.6

Figure 2.

Figure 2

(A) Significant correlation of B-cell-activating factor (BAFF) with total (106 cells) inflammatory cells. Cell viability was determined by means of trypan blue dye exclusion, and differential cell counts were obtained by using a Diff-Quik stain (American Scientific Products, McGaw Park, IL) of cytocentrifuge preparations (Cytospin; Shandon Southern Instruments, Inc., Sewickley, PA). Correlations were assessed with the Spearman rank correlation. Significant correlation was observed between BAFF level and bronchoalveolar lavage total inflammatory (106) cells (r = 0.823, p = 0.001). The concentrations of BAFF detected in BAL fluid were highly correlated with the absolute numbers of recruited inflammatory cells. (B) Significant correlation of B-cell-activating factor (BAFF) with bronchoalveolar lavage (BAL) macrophages (106 cells). Differential cell counts were obtained by using Diff-Quik staining of cytocentrifuge preparations. Correlations were assessed with the spearman rank correlation. Significant correlation was observed between BAFF level and BAL macrophages (r = 0.742, p = 0.001). The concentrations of BAFF detected in BAL fluid were highly correlated with the absolute numbers of macrophages. (C) Significant correlation of B-cell-activating factor (BAFF) with bronchoalveolar lavage (BAL) lymphocytes (106 cells). Differential cell counts were obtained by using Diff-Quik staining of cytocentrifuge preparations. Correlations were assessed with the spearman rank correlation. Significant correlation was observed between BAFF level and BAL lymphocytes (r = 0.709, p = 0.001). The concentrations of BAFF detected in BAL fluid were highly correlated with the absolute numbers of recruited inflammatory lymphocytes. (D) Significant correlation of B-cell-activating factor (BAFF) with bronchoalveolar lavage (BAL) neutrophils (106 cells). Differential cell counts were obtained by using Diff-Quik staining of cytocentrifuge preparations. Correlations were assessed with the Spearman rank correlation. Significant correlation was observed between BAFF level and bronchoalveolar lavage fluid (BAL) neutrophils (r = 0.809, p = 0.001). The concentrations of BAFF detected in BAL fluid were highly correlated with the absolute numbers of recruited neutrophils.