Figure 4.

Microinjection of bicuculline into the PnO blocked the increase in wakefulness, decrease in NREM sleep, and decrease in REM sleep caused by muscimol. A–I, *p < 0.05, significant difference from control (saline). Data summarize the results from 4 h of recordings. J, A coronal diagram at 4.60 mm posterior to bregma was modified from a mouse brain atlas (Paxinos and Franklin, 2001) to indicate the mean ± SEM location of 12 histologically confirmed microinjection sites (black dot and bars in left PnO).