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. 2010 Mar 4;16(5):1769–1779. doi: 10.1089/ten.tea.2009.0625

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 3. — Smooth muscle- and urothelial-specific protein expression on human BMSCs differentiated on day 14. (A) Lane 1, fresh smooth muscle tissue; lane 2, cultured SMCs as positive controls; lane 3, cultured urothelium as negative control; lane 4, noninduced BMSCs as control; lane 5, induced BMSCs by coculture with SMCs; lane 6, induced BMSCs by SMC-CM. The housekeeping gene, β-actin, was used as the load control. (B) Lane 1, bladder urothelium as control (p3); lane 2, noninduced BMSCs (p3); lane 3, BMSCs cocultured with urothelium; lane 4, BMSCs cultured with UC-CM. AE1/AE3, antibodies against cytokeratins; CK-13, cytokeratin-13, Up-Ia, Uroplakin-Ia.