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Myogenic differentiation of human BMSCs using SMC-derived CM. Human BMSCs (p4) were stained with α-SMA (a, e, i), calponin (b, f, j), desmin (c, g, k), and myosin (d, h, l) antibodies without induction as negative control (a–d) and with induction for 14 days (e–h). SMCs were also stained with the same antibodies as a positive control (i–l). Scale bar = 50 μM. Color images available online at www.liebertonline.com/ten.
