FIG. 5.

Urothelial differentiation of human BMSCs using coculture approach. Top panel: (i, ii) noninduced BMSCs as negative control (iii, iv) BMSCs induced to urothelial differentiation by coculture with urothelium; (v, vi) urothelial differentiation of BMSC by UC-CM; (vii, viii) urothelial cells (p4) as positive controls. Cells were stained with urothelial-specific markers Up-Ia and AE1/AE3 (pancytokeratin). The secondary antibody was conjugated to fluorescein isothiocyanate (green) and nuclei counterstained using propidium iodide (red). Scale bar = 50 μM. Color images available online at www.liebertonline.com/ten.