FIG. 7.

Muscle tissue contractility following myogenic differentiation of human BMSCs. BSMs were seeded with human BMSCs and were continuously stretched (5 pulls/min) to 10% of their original length for 8 days. Contractility response of human BMSC-seeded BSM: (left) in vitro and (right) in vivo. Organ baths were used to study contractility of muscle-induced human BMSCs by subjecting them to an electrical field stimulation of 5 V, 1 Hz. Seeded BSMs were cultured in vitro using SMC-CM (stretch and static) or standard culture medium (control) for 14 days and assayed for contractility or in vivo implanted into nude mice and assayed after 4 weeks. Nude mice used for in vitro studies (n = 4) and in vivo studies (n = 3). *p < 0.05 (t-test), compared with static culture. BSMs, bladder submucosal matrixes.