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. 2010 Mar 4;16(5):1769–1779. doi: 10.1089/ten.tea.2009.0625

FIG. 8.

FIG. 8.

Muscle tissue formation in vivo. (A) Bladder extracellular matrix and (B) PLLA. Cross sections of the implanted scaffold were retrieved from the nude mice after 1 month. (A-a) Immunofluorescence analysis for DAPI (4′,6-diamidino-2-phenylindole) and (A-b) Masson's trichrome, and (A-c) immunohistochemistry analysis for desmin staining of cell-seeded bladder extracellular matrix implants. Images are shown at 400 ×. (B) Immunohistochemistry staining of cell-free PLLA implant (B-a, B-b), SMC-seeded PLLA as a positive control (B-c, B-d), noninduced BMSC-seeded PLLA as negative control (B-e, B-f), and PLLA seeded with BMSCs cultured using SMC-CM for 14 days (B-g, B-h). Hematoxylin and eosin-stained sections (on the left) are shown at 200 × and desmin-stained sections (on the right) at 400 ×. PLLA, poly-l-lactic acid. Color images available online at www.liebertonline.com/ten.