FIG. 6.

Conditioned medium samples collected every 3.5 days were analyzed for sGAG content and normalized to joint surface area. Joints were incubated with (filled) or without (open) 0.5 mM cycloheximide (CX) at 4°C, 37°C, or for 7 days at 4°C and then 7 days at 37°C (4/37°C) (A). Within each time point, letters (a, b, and c) designate significantly different groups (p < 0.05), with a shared letter indicating no difference. *Initial time point not different from second point, but different from the remaining time points (p < 0.05), **Initial time point different from the remaining time points (p < 0.05). Average sGAG release for each group over 0–7 days (B) and 7–14 days (C) was normalized to the sGAG release from the 37–CX group from 0 to 7 days to emphasize differences due to CX and temperature.