FIGURE 3.

Complementation of yeast by orthologous eIF4E proteins. Plasmid shuffling was carried out with haploid yeast strain RH2585, which carries the deletion Δcdc33::kanX, and the essential eIF4E activity is provided on plasmid pVTURA3. Cells were transformed with plasmids carrying different eIF4E versions (yeast, Drosophila, murine, and vector) and plated on yeast minimal medium containing 5-fluoroorotic acid to counterselect for the loss of the eIF4E-encoding plasmid. Note that Drosophila eIF4E and murine eIF4E complement the lack of yeast eIF4E, but murine eIF4E-transformed yeast cells show a slow growth phenotype.