. 2010 Aug 13;285(42):31923–31929. doi: 10.1074/jbc.M110.152447

TABLE 2.

Incorporation of [³⁴S]sulfide into Fe-S clusters and cysteinyl and methionyl residues in the M. maripaludis ΔsepS mutant

Sulfur sources in the growth medium^a	Atom % of ³⁴S
Sulfur sources in the growth medium^a	Fe-S	Cys	Met
[³²S]Cys + [³⁴S]Na₂S^b	87 ± 4	8 ± 2	92 ± 3
[³²S]Cystathionine + [³⁴S]Na₂S^c	84	5	5
[³²S]Cys + [³²S]Hcy + [³⁴S]Na₂S^d	90	9	7

^a Cells were grown in media containing 1.5 mm [³⁴S]sulfide, 3 mm l-cysteine, 5 mm l-cystathionine, and/or 3 mm l-homocysteine as indicated. The numbers represented the measured atom % of ³⁴S in the derivatives of the indicated molecules. The ³⁴S enrichment of Na₂S used as the substrate was 92.6%. The natural abundance of ³⁴S in the sulfur-containing amino acids was 4.2%.

^b Values are the means of three independent experiments ± 1 S.D.

^c Medium contained 1.5 mm coenzyme M as a reductant.

^d Values are the means of two independent experiments.