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. 2010 Aug 18;285(42):31954–31964. doi: 10.1074/jbc.M110.148718

FIGURE 5.

FIGURE 5.

Identification of lysines acetylated by p300. A, MALDI-TOF MS spectrum of histone H4 peptides from activator-dependent p300-acetylated HTLV-1 chromatin templates is shown. Starred peaks (*) indicate matches to predicted H4 peptides; acetylated peptides are labeled as indicated. B, shown is the MALDI-TOF MS spectrum of histone H3 peptides from activator-dependent p300-acetylated HTLV-1 chromatin templates. Starred peaks (*) indicate matches to predicted H3 peptides; acetylated peptides are labeled as indicated. C, the MS/MS spectrum of peptide 27KACSAPATGGVKACKACPHR40 (amino acids 27–40 of X. laevis H3 sequence) confirm acetylation on lysines 27, 36, and 37. Confirmation was based on the Mascot ion score of 104 and coverage of theoretical y and b ions including the sequence region of acetylated Lys-27, -36, and -37. The peak at m/z 126 corresponds to an acetylated lysine immonium ion, NH3, which further confirms the presence of acetylated lysine. Peaks corresponding to internal fragmentation are unlabeled shown in light gray.