FIGURE 1.

SIRT4 knockdown increase FAO gene expression and FAO in mouse primary hepatocytes. Primary hepatocytes cells were silenced with a shRNA-SIRT4 adenovirus. A, quantitative real time PCR showing that SIRT4 was decreased by shRNA-SIRT4 compared with shRNA-control cells. B–F, mouse primary hepatocytes were transduced with control or shRNA-SIRT4 adenovirus. Cells were harvested after 48 h. RNA was extracted and used to measure the indicated gene expression using quantitative real time PCR analysis. G, FAO in control and SIRT4 knockdown cells was measured. Values represent the mean of quadruplicate samples. Error bars represent S.E. Statistical analyses were performed using analysis of variance. *, p < 0.05.