FIGURE 4.

SIRT4 knockdown increases fatty acid oxidation genes in mouse liver. Mice were infected with shRNA-control or shRNA-SIRT4 adenovirus. Animals were sacrificed 4 days after adenovirus injection. Liver RNA was extracted and used to measure the indicated gene expression using quantitative real time PCR analysis (A), body weight (B), liver weight (C), and blood glucose (D). Data are presented as mean ± S.E. n = 5/group. Statistical analyses were performed using Student's t test. *, p ≤ 0.05.