FIGURE 2.

AKT mediates fMLP-induced activation of ERK but not p38 MAPK in dHL-60 cells. A and B, time course of ERK phosphorylation induced by 1 μm fMLP in dHL-60 cells that were pretreated without (control) or with 3 μm AKTib1/2 for 15 min. C–F, concentration-dependent effects of 0.5–10 μm AKTib1/2 on the phosphorylation of ERK1/2 (C and D) and p38 MAPK (E and F) induced by fMLP (1 μm, 1 min) in dHL-60 cells. Representative Western blots are shown in A, C, and E, and densitometric quantification is expressed as a percentage of the respective control in B, D, and F (n = 4 experiments).