FIGURE 7.

AKT activation is required for fMLP-mediated RB and translocation of NOX components but not for PMA-induced RB. A and B, dHL-60 cells pretreated without (control) or with 0.25–3 μm AKTib1/2 (A) or with 10 nm wortmannin (B) for 15 min before stimulation with 1 μm fMLP or 0.5 μm PMA for 5 min. Superoxide production is expressed as a percentage of control values (five experiments). C, representative Western blot of p47^phox, p67^phox, ERK, AKT, phospho-p47^phox (P-p47^phox), phospho-ERK1/2 (P-ERK), and phospho-AKT (P-AKT) in the membrane fractions of dHL-60 cells that were pretreated in the absence or presence of AKTib1/2 before stimulation with 1 μm fMLP for 2 min. D, amount of proteins translocated at the membrane in fMLP-stimulated cells quantified and expressed as a percentage of stimulated control values (n = 3 experiments). E, phosphorylated form of p47^phox, ERK, and AKT quantified and expressed as percentage of stimulated control values (n = 3 experiments). *, p < 0.05.