FIGURE 9.
Effect of antisense oligonucleotides on AKT1 and AKT2 on fMLP-mediated PLD activity, ERK phosphorylation, and RB in dHL-60 cells. Cells were transfected with 8 μm antisense oligonucleotides with AKT1 (AS-T1) or AKT2 (AS-T2) or sense (control). A, expression of AKT1, AKT2, and p47phox in transfected dHL-60 cells, revealed by Western blotting. B–E, RB (B), PLD activity (C), and phosphorylation of ERK (D and E) were stimulated with 1 μm fMLP. Significant differences between control and antisense-treated cells are indicated by * p < 0.05. Blots are representative of three experiments.