FIGURE 5.

A, regeneration of oxidation activities in rH-1, rH-2, and WT SSF as normalized initial rates. Each injection is half-saturation of the ferroxidase sites, 1 Fe²⁺/site. Conditions were as follows: [aporH-1, aporH-2, or WT apoSSF] = 2.0 μm in 0.15 m NaCl and 100 mm Mops, pH 7.0, 25 °C, time intervals between injections 3 min. Error bars, S.E. B, change of relative fluorescence intensity of apoferritin after the aerobic addition of 48 Fe²⁺ atoms/shell. Conditions were as follows: λ_ex = 280 nm, λ_em = 325 nm, [aporH-1, aporH-2, or WT apoSSF] = 0.5 μm in 0.15 m NaCl and 0.1 m Mops, pH 7.0, 25 °C.