TABLE 1.
Phenotypes of mutants of the B. pseudofirmus OF4 a-subunit that are conserved in mesophilic alkaliphiles but not in C. thermarum TA2.A1
The growth results are from two independent experiments carried out in duplicate. The OG ATPase and β-subunit content assays were done on two independent everted membrane vesicle preparations. The values are percent of wild-type ± S.D.
| Strain | Glucose |
Malate |
OG ATPase | β-Subunit content | ||
|---|---|---|---|---|---|---|
| pH 7.5 | pH 10.5 | pH 7.5 | pH 10.5 | |||
| Wild-type | 100 | 100 | 100 | 100 | 100 | 100 |
| aE98S | 92.4 ± 0.8 | 95.0 ± 12.8 | 80.0 ± 0.7 | 44.1 ± 7.3 | 124.3 ± 4.4 | 114.4 ± 3.8 |
| aM171L | 91.7 ± 2.4 | 100.6 ± 10.2 | 78.0 ± 2.9 | 49.0 ± 8.6 | 97.3 ± 5.9 | 122.0 ± 12.7 |
| aV177I | 73.0 ± 3.9 | 52.8 ± 4.2 | 54.7 ± 3.2 | 31.9 ± 1.6 | 83.9 ± 10.2 | 83.0 ± 14.5 |
| aM184L | 78.5 ± 10.2 | 62.0 ± 13.3 | 29.8 ± 6.1 | 20.4 ± 9.2 | 100.7 ± 8.8 | 103.4 ± 10.5 |
| aI185G | 81.0 ± 8.8 | 74.9 ± 17.4 | 83.6 ± 4.6 | 48.6 ± 14.8 | 69.2 ± 1.9 | 57.8 ± 10.8 |
| aI185S | 73.4 ± 5.4 | 64.1 ± 14.6 | 71.8 ± 5.8 | 47.5 ± 10.9 | 64.8 ± 14.0 | 48.2 ± 6.5 |
| aL205M | 91.2 ± 8.0 | 85.8 ± 13.7 | 33.2 ± 6.4 | 29.6 ± 9.6 | 80.3 ± 6.2 | 87.0 ± 26.7 |