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. 2010 Jul 30;285(42):32126–32140. doi: 10.1074/jbc.M110.113332

FIGURE 8.

FIGURE 8.

Apparent time-course of modification of activated and non-activated T338C channels by internal Au(CN)2−. Mean effect of internal MTSES (200 μm) on macroscopic current amplitude at −80 mV after maximal channel activation with PKA (20 nm), ATP (1 mm), and PPi (2 mm). Patches were pretreated with Au(CN)2− alone (1 mm) (A) or Au(CN)2− (2 μm) in the presence of PKA (20 nm) and ATP (1 mm) (B), for variable periods of time indicated on the abscissa. Data for pretreatment with Au(CN)2−, PKA, and ATP have been fitted by an exponential function to estimate the rate of modification of activated channels. For pretreatment of non-activated channels, there was no significant change in apparent MTSES sensitivity even after pretreatment with 1 mm Au(CN)2− for 5 min (p > 0.5), suggesting that no significant degree of Au(CN)2− modification had taken place in these channels during the pretreatment period. Data are the means from 3–4 patches at each pretreatment time.