FIGURE 2.

Positional cloning of the Pgu mutation. A, high resolution haplotype mapping of Pgu mutation on mouse chromosome 3 (Chr. 3) is shown. White squares indicate C3H homozygotes; gray squares indicate B6/C3H heterozygotes. The SNPs defining the minimal interval are shown in bold text. Map positions are in accordance with the public mouse genome assembly (Ensembl). B, shown is sequencing of mouse candidate Kcna2 cDNA revealed a T-to-C transition at nucleotide residue 1886, leading an isoleucine to threonine substitution at residue 402 in Pgu/+ and Pgu/Pgu mice. C, shown is a protein sequence alignment of the S6 transmembrane domain (bold text) of the voltage-gated potassium channel Kv1 subfamily in human and mouse genome. The Pgu I402T mutation is indicated by an arrow.