FIGURE 7.

Inhibition of human kallikreins by rSPINK6. Inhibition of KLKs (KLK5, KLK7, KLK8, and KLK14) was performed using increasing amounts of rSPINK6. The reaction progress was monitored by measuring the absorbance at 405 nm_. Initial reaction velocities for each tested inhibitor concentration were determined by linear regression and were subsequently plotted against inhibitor concentration. Data were further analyzed according to the Morrison tight-binding inhibition model. The graphs include the means ± S.E. of at least three replicates for each enzyme.