TABLE 1.
Protease inhibition by recombinant SPINK6
pNA, p-nitroanilide.
| Proteinase | SPINK6 | Inhibition | Substrate (0.33 mm) |
|---|---|---|---|
| nm | % | ||
| Bovine trypsin (2 nm final concentration) | 400 | 0 | N-(p-Tosyl)-Arg-Gly-Val 5-NA |
| Cathepsin G (1 nm final concentration) | 666 | 0 | N-Succinyl-Ala-Ala-Pro-Phe pNA |
| Chymase (2 nm final concentration) | 666 | 0 | N-Succinyl-Ala-Ala-Pro-Phe pNA |
| Human chymotrypsin (2 nm final concentration) | 400 | 0 | 3-MeO-Arg-Pro-Tyr pNA |
| KLK14 (2 nm final concentration) | 400 | 99.9 | N-(p-Tosyl)-Arg-Gly-Val 5-NA |
| KLK5 (5.3 nm final concentration) | 400 | 99.9 | N-(p-Tosyl)-Arg-Gly-Val 5-NA |
| KLK7 (15.8 nm final concentration) | 400 | 88.3 | MeO-Suc-Arg-Pro-Tyr pNA |
| Human leukocyte elastase (2 nm final concentration) | 400 | 0 | N-MeO-Ala-Ala-Pro-Val pNA |
| Human plasmin (2 nm final concentration) | 400 | 0 | N-(p-Tosyl)-Gly-Pro-Lys 4-NA |
| Human thrombin (1 nm final concentration) | 400 | 0 | N-(p-Tosyl)-Gly-Pro-Arg pNA |
| Matriptase (0.5 nm final concentration) | 400 | 0 | H-d-Ile-Pro-Arg pNA |
| Prostasin/Prss8 (3.5 nm final concentration) | 1750 | 3.3 | Tosyl-Gly-Pro-Arg 7-amino-4-methylcoumarin |