TABLE 3.

Effects of SPINK6 on cell shedding from human plantar callus

Single 3-mm callus cylinders (∼0.5 mm thick) were incubated for 18 h at 37 °C in 0.1 m Tris-HCl and 5 mm EDTA (pH 8.0) with protease inhibitors as indicated.

Inhibitor	Inhibition ± S.E.	n
	%
Buffer	0 ± 12.9	5
SPINK6 (10 μm)	98.7 ± 0.5	5
SPINK6 (5 μm)	90.7 ± 2	2
SPINK6 (2.5 μm)	78.1 ± 8.8	2
Aprotinin (10 μm)	96.4 ± 2.2	3
α-Antitrypsin (10 μm)	95 ± 3.7	3