TABLE 1.

S. cerevisiae strains carrying rdn mutations and their phenotypes

Mutation	18 S rRNA base change	Altered domain	Helix altered	Subunit bridge affecteda	Plasmid introduced	GCN2+ strainsb	his4-306(TTG) strainsc	Gcnd	Ssue
WT	Wild-type				pNOY373	NOY908, KAY761	KAY165	+	+
rdn-41	A952C	Central	22 bulge	B2e	pNOY373-41	KAY498, KAY757	KAY428	–	+
rdn-41a	A952C	Central	h22 bulge	B2e	pNOY373-41a	KAY906	NC	–	NT
rdn-42	A1193U	3′-Major	h31 loop		pNOY373-42	KAY767	KAY429	–	–
rdn-44	G875A	Central	h22 bulge	B2e	pNOY373-44	KAY776	KAY431	–	–
rdn-59	A333G	5′	h11 loop	B4 and/or eB10	pNOY373-59	KAY575, KAY769	KAY449	–	+
rdn-59a	A333G	5′	h11 loop	B4 and/or eB10	pNOY373-59a	KAY907	NC	–	NT
rdn-60	A138G	5′	Junction of h7/10 and h8/9		pNOY373-60	KAY500, KAY778	KAY455	±	+
rdn-61	G322A	5′	h11 bulge	B4 and/or eB10	pNOY373-61	KAY501, KAY770	KAY450	±	+
rdn-63	ΩG1112	Central	h27 bulge		pNOY373-63	KAY502, KAY780	KAY451	±	+
rdn-71	G362A	5′	h12 stem		pNOY373-71	KAY546, KAY760	KAY462	±	+
rdn-72	G922A	Central	h23 stem	B2d/e	pNOY373-72	KAY547	KAY463	±	+
rdn-76	G1108A	Central	bottom of h27		pNOY373-76	KAY549, KAY768	KAY494	±	+

^a Intersubunit bridges potentially affected, based on cryo-EM structures with subatomic resolutions (15–17). B, universally conserved bridge; eB, Eukarya-specific bridge.

^b Derivatives of NOY908 (MATa ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1 can1-100 rdnΔΔ::HIS3 pNOY373 [2μ RDN LEU2]) carrying indicated mutations.

^c Derivatives of KAY165 (MATa ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1 can1-100 rdnΔΔ::HIS3 his4-306(TTG) pNOY373 [2μ RDN LEU2]) carrying indicated mutations. NC, not constructed for this study.

^d Gcn phenotype as judged by the experiment in Fig. 5A. −, reduced viability as well as reduced colony growth in the presence of 3AT; ±, reduced colony growth only.

^e Ssu phenotype as judged by the experiment in Fig. 5C. NT, not tested.