FIGURE 5.

Effect of 5′ splice site mutations on the alternative processing of hERG1 mRNA. A, schematic representation of the 5′ splice site sequence and mutations. B, RPA analysis of the minigenes carrying the wild-type (WT), 5′ splice site mutation +4U>A/+6G>U (+4+6), canonical (Can) intron 9 poly(A) signal, and +4U>A/+6G>U plus canonical poly(A) signal mutation (+4+6/Can). The expression level of the hygromycin B resistance gene served as the loading control. Results shown are representative of three independent experiments.