FIGURE 6.

Microtubule detachment during mitosis. A, EGFP-MAP4-transfected CHO cells were left untreated (upper panels) or were treated 30 min with 15 nm vinblastine (lower panels) and were then viewed by live cell fluorescence microscopy. Prophase cells were located and photographed every 5 s. The images were deconvolved to improve contrast. Arrows point to the minus-ends of microtubules that detached from the spindle poles. Bar = 5 μm. B, the number of detachments seen in control- and drug-treated prophase and interphase cells were counted from captured images and divided by the total time of observation. The mean ± S.D. was calculated from 11–20 cells during a total observation time of 50–80 min.