TABLE 1.
Effect of vinblastine on microtubule dynamics in prophase CHO cells
Values shown represent the mean ± S.E.
| Interphase |
Prophase |
|||
|---|---|---|---|---|
| 0 | 0 | 5 | 35 nm | |
| Growth | ||||
| Rate (μm/min) | 16.2 ± 0.8 | 17.1 ± 1.6 | 9.9 ± 0.7a | 12.3 ± 1.0a |
| Distance (μm) | 3.2 ± 0.4 | 2.5 ± 0.3 | 0.9 ± 0.1a | 1.3 ± 0.1a |
| Duration (sec) | 12.2 ± 1.2 | 9.0 ± 0.6 | 5.4 ± 0.3a | 6.4 ± 0.3a |
| Shortening | ||||
| Rate (μm/min) | 28.2 ± 2.3 | 24.6 ± 2.0 | 13.5 ± 1.5a | 13.9 ± 0.9a |
| Distance (μm) | 5.2 ± 0.6 | 3.3 ± 0.4 | 1.4 ± 0.1a | 1.6 ± 0.1a |
| Duration (sec) | 11.2 ± 1.1 | 8.7 ± 0.9 | 6.3 ± 0.3a | 6.7 ± 0.3a |
| % Time | ||||
| Growth | 31.1 ± 2.2 | 36.0 ± 2.4 | 12.2 ± 1.8a | 15.9 ± 2.1a |
| Shortening | 21.1 ± 1.9 | 33.0 ± 2.8 | 14.0 ± 0.8a | 16.3 ± 1.7a |
| Pause | 47.8 ± 3.1 | 31.0 ± 3.9 | 73.8 ± 3.9a | 67.7 ± 3.3a |
| Frequency (min−1) | ||||
| Catastropheb | 1.5 ± 0.1 | 3.4 ± 0.3 | 1.6 ± 0.1a | 1.8 ± 0.2a |
| Rescuec | 6.3 ± 0.5 | 6.0 ± 0.5 | 9.3 ± 0.9a | 9.4 ± 0.5a |
| Dynamicityd (μm/min) | 9.0 ± 0.9 | 13.8 ± 1.4 | 3.1 ± 0.4a | 4.2 ± 0.6a |
| Number of microtubules | 30 | 12 | 8 | 15 |
a p < 0.05 compared to 0 concentration in prophase cells.
b The transition from growth or pause to shortening.
c The transition from shortening to growth or pause.
d The total change of length per unit time of individual microtubules during their life histories.