FIGURE 6.

Binding of EspP-containing ropes to HeLa Cells. A, HeLa cells (red) alone. B and C, HeLa cells incubated with ropes (B) or EspP (C) (green) obtained from DH5α(pB9-5) after 24 h of incubation. In both cases note the loss of stress fibers and retraction of cell bodies indicating cytopathic activity. Photomicrographs (A–C) were taken at 60×. D, kinetics of adherence of EspP versus ropes to HeLa cells. E, cytopathic effects of EspP or ropes on HeLa cells were quantified using immunofluorescence microscopy counting the number of cells with morphological changes (e.g. cell rounding and loss of plasmic membrane) compared with the control without EspP or ropes.