FIGURE 7.

US2-mediated retrotranslocation is independent of Ufd1. a, 293T cells stably expressing TCRα or US2 were transfected with either control siRNA or siRNA targeting p97 or Ufd1. Whole cell extracts were analyzed by immunoblotting with antibodies against the indicated proteins. The number indicates the relative intensity of the Ufd1 band. Note that the GFP antibody also detects a nonspecific protein in US2 cell extract, which migrates at the same position as TCRα-YFP. b, shown are normalized HC levels in US2 cells transfected with the indicated siRNAs from three independent experiments. Error bars represent S.E. (n = 3).