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. 2010 Aug 16;285(42):32360–32369. doi: 10.1074/jbc.M110.131045

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Maspin disrupted focal adhesion disassembly. A, immunofluorescence staining against vinculin showed abundant formation of focal adhesion in starved HUVECs following nocodazole treatment for 4 h (10 nm). Time point is set as 0 min before nocodazole washout. B, vehicle-treated HUVECs formed very few focal adhesions. Panels C, E, G, and I, vinculin staining exhibited dynamic changes of focal adhesions in GST-Maspin-treated HUVECs at the indicated time points. Panels D, F, H, and J, corresponding alteration of focal adhesions in the GST control group. K, Western blotting analysis showed the alteration of the phosphorylated FAK (Y397) level in HUVECs during focal adhesion disassembly. Total FAK served as a loading control. Note that the level of Tyr(P)³⁹⁷-FAK was relatively higher in GST-Maspin-treated HUVECs compared with GST-treated HUVECs.