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. 2010 Aug 9;285(42):32370–32377. doi: 10.1074/jbc.M110.144774

FIGURE 1.

FIGURE 1.

Neural agrin induces a rapid but transient activation of ERK1/2 in C2 cells. Time course of ERK1/2 activation following application of 0.1 nm recombinant neural agrin to C2 myotubes. Top panel, Western blot probed with antibodies to active, phosphorylated ERK1/2 (pERK1/2). Bottom panel, same Western blot probed with antibodies to total ERK1/2 (tERK1/2). ERK1/2 were strongly activated within 2 min of neural agrin application. They peaked after 5–10 min and returned to control by 60 min. The arrowheads on the left side of the top panel point to ERK1 (p44) and ERK2 (p42). This blot was developed using film. This experiment was repeated once with similar results.