FIGURE 4.

Fog2 knockdown impairs adipogenesis. 3T3-L1 cells were transfected with either shRNA constructs targeting Fog2 (shRNA1 and shRNA2) or a scrambled shRNA or subjected to mock transfection. A, cells were harvested at 2 days postconfluence, and the level of Fog2 mRNA was assessed by real-time PCR. Expression of Fog2 was normalized with 18S and expressed relative to the mock transfected level. Error bars, S.E. (n = 3). B, Western blot of FOG2 knockdown. Cells were harvested prior to differentiation, and nuclear extracts were prepared. Western blotting was performed with a FOG2 antibody, and GAPDH antibody was included as a loading control. C, images of 3T3-L1 cells transfected with shRNA constructs at day 0 and day 5 of differentiation. Cells were imaged using light microscopy. Scale bars, 20 μm.