FIGURE 6.

Mutation of the FOG CTBP binding site impairs FOG repression of adipogenesis. A, Western blot of FOG2 and FOG2-ΔDL overexpression in 3T3-L1 cells. Retroviral delivery was used to express FOG2 and FOG2-ΔDL in 3T3-L1 preadipocytes. Cells were grown to 2 days postconfluence, and nuclear extracts were prepared. Western blotting was performed with a FOG2 antibody. Blotting with GAPDH antibody was included as a loading control. B, images of 3T3-L1 cells expressing FOG2 and FOG2-ΔDL after 5 days of differentiation. Cells expressing FOG2, FOG2-ΔDL, or an empty vector control were induced to differentiate for 5 days. Cells were imaged using light microscopy. Scale bars, 20 μm. C, Oil Red O staining of 3T3-L1 cells expressing FOG2 and FOG2-ΔDL after 5 days of differentiation. Cells were stained with Oil Red O and imaged by scanning. The stain was extracted and quantified by spectrophotometry. Results are expressed as staining relative to control. Error bars, S.E. (n = 3).