TABLE 3.

SAXS data collection, processing and modelling statistics

	pH 5	pH 8
P(r) function calculation
q range	0.018–0.464 Å−1	0.021–0.464 Å−1
Rg	35.0 ± 0.2 Å	36.2 ± 0.1 Å
I(0)	88.5 ± 0.3	108.4 ± 0.3
Dmax	127 Å	128 Å
Estimated molecular massa	31 kDa	38 kDa
Mass calculated from sequence	37 kDa	37 kDa
Ab initio GASBOR modeling
Ensemble average χ2 to raw data	1.98 ± 0.07	2.15 ± 0.07
NSDb	1.11 ± 0.04	1.47 ± 0.07

^a Normalization against data for BSA was calculated using the formula (I(0)_FAP ÷ I(0)_BSA·66 kDa), where I(0)_BSA was 188.3.

^b For the definition of normalized spatial discrepancy (NSD) (88).