FIGURE 2.

Effects of bSMase on sphingomyelin in FBS medium. A, the SL profile was measured in FBS medium, and different species of SM were detected. B, after bSMase treatment (25 milliunits (mU)/ml), ceramide formation was measured. Only the C₁₆ acyl chain is shown for clarity, but all sphingomyelin species had similar kinetics. C, HeLa cells were cultured in 10% FBS-containing DMEM medium or FBS-free medium and treated with bSMase (25 milliunits/ml for 10 min), and phospho-medium and total ezrin were measured by Western blotting.