FIGURE 2.

Middle domain mutations decrease Drp1 recruitment/retention on mitochondria. A, expression of the Myc-tagged wild-type (WT) and mutant forms of Drp1 was equal. Actin levels were monitored as a loading control. B, HeLa cells transfected with WT Myc-Drp1, or else the indicated Drp1 mutants were either left untreated or treated with staurosporine (STS) in the presence of the broad caspase inhibitor benzyloxycarbonyl-VAD-fluoromethyl ketone. Levels of Drp1 in the mitochondrial fraction were assessed by immunoblotting, with levels of the mitochondrial protein HSP60 monitored as a loading control.