FIGURE 1.

Constitutive high NF-κB activity in OSCC cell lines. The gel shift was performed with nuclear extracts from both control and OSCC cells. The cells were incubated with ³²P-labeled κB oligonucleotide probe. A, there is clear binding of the probe to the nuclear extracts from OSCC9 and OSCC25 cells but not in HOK cells. B, a gel-shift assay in OSCC25 is shown. p50, and to a lesser extent p65, are present in the NF-κB complex and can be super-shifted by antibodies specific to p50 and p65. The addition of excess cold NF-κB oligonucleotide probe competes out the formation of the NF-κB complex, demonstrating the specificity of NF-κB binding. Arrowheads mark the NF-κB complex.