FIGURE 3.

Palmitate induces early ACLY phosphorylation that does not impact ACLY activity. Phosphorylation of ACLY at serine 454 (A) and threonine 447/serine 451 (B) were determined following treatment of MIN6 cells for 24 h with palmitate (1500 μm; 6:1 ratio versus BSA) and with 5 or 25 mm glucose as indicated. Shown is the time course of changes in total ACLY (C) and phosphorylated ACLY (serine 454) (D) in MIN6 cells exposed to palmitate (1500 μm, 6:1 ratio versus BSA) in 5 or 25 mm glucose for the indicated time points up to 6 h. *, significant difference from the control of the same glucose condition; **, significant difference between the controls at different glucose conditions. Error bars, S.E.