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. 2010 Aug 13;285(42):32671–32677. doi: 10.1074/jbc.M110.128256

FIGURE 2.

FIGURE 2.

Expression of mutant Sss1p derivatives and SEC translocon subunits. A, expression of Sss1p mutants. Whole cell extracts prepared from FKY198 cells transformed with vector, wild-type SSS1 (WT), Sss1ΔC, and NSU plasmids grown in galactose (−Glu) and after transfer to glucose medium for 6 h (+Glu) were subjected to 12.5% SDS-PAGE and immunoblot analysis with Sss1p antiserum. The panels were edited from the same immunoblot. B, whole cell extracts prepared from FKY198 cells after transfer to glucose medium for 6 h bearing wild-type and USC plasmids as indicated were analyzed by 10% SDS-PAGE and immunoblotting with Ubc6p antiserum. C, glucose-repressed extracts from above (A) were analyzed by 12.5% SDS-PAGE and immunoblotting with antiserum against Sec61p, Sec62p, Sec63p, and Sbh1p as indicated. These data are representative of two independent experiments.