FIGURE 4.
NSUp interaction with Sec61p. Membranes prepared from CMY28 cells transformed with the pRS316-based SSS1/NSU plasmids or vector before (lanes 1, 2, 9, and 10) and after 10-h growth in 2 mm methionine medium (lanes 3–8, 11–16) were mock-treated or treated with 2 mm Cu2+(phe)3 (Phe). After 13% nonreducing SDS-PAGE, immunoblotting with Sss1p (left panel) and reprobing of the same blot with Sec61p (right panel) antiserum was performed. The adduct formed between Sec61p-TM9C3 and Sss1p/NSUp is marked as x-link. These data are representative of two independent experiments.