Figure 5.

β₁-Adrenoceptor protein and gene expression in cultured sympathetic neurons. A, mRNA extracted from purified SCG neuronal cultures contains sequences corresponding to the β₁-adrenoceptor gene, as revealed by RT-PCR. Ventricular myocardium was used as a positive control, and GAPDH was used to show the relative level of receptor expression. B, C, Immunostaining for β₁-adrenoceptor show that this protein is present in sympathetic neurons (B), as revealed by peripherin staining (C). Inset in B shows a glial cell that is negative for β₁-AR immunoreactivity. D, A merged image shows that β₁-adrenoceptor protein is present throughout the soma and processes including axon terminations. Scale bar, 10 μm.